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A photostable monomeric superfolder GFP.

Valbuena FM, Fizgerald I, Strack RL, Andruska N, Smith L, Glick BS
Traffic. 2020 May 15. doi: 10.1111/tra.12737. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
135301pQE-60NA-msGFP2Encodes a photostable monomeric superfolder GFP
160459pQE-60NA-mEGFPEncodes a monomeric enhanced GFP variant
160460pQE-60NA-mCherryEncodes mCherry
160461pQE-60NA-mCherry2CEncodes an mCherry variant that displays lower cytotoxicity in bacteria
160462pQE-60NA-mScarlet-IEncodes the original mScarlet-I
160463pQE-60NA-mScarlet-2IEncodes a variant of mScarlet-I that displays reduced cytotoxicity in bacteria
160464pQE-60NA-proinsulin-mEGFPEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria
160466pQE-60NA-proinsulin-msGFP2Encodes a fusion of human proinsulin and the monomeric superfolder GFP2
160467YIplac211-KAR2-mEGFP-HDELYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal
160468YIplac211-KAR2-msGFP2-HDELYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal
160469pUC19-PpSEC13-mEGFPP. pastoris integration vector for the gene replacement of SEC13 fused to mEGFP
160470pUC19-PpSEC13-msGFP2P. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2
160471yo-msGFP2Encodes a yeast codon optimized version of msGFP2

Antibodies from Article