A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants.
Alcantara A, Seitner D, Navarrete F, Djamei A
Plant Methods. 2020 Jan 21;16:4. doi: 10.1186/s13007-020-0552-3. eCollection 2020.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135231 | pBIP1::eYFP | UPR signaling reporter (upregulates eYFP expression under UPR conditions) |
| 135232 | p35S::IRE1a | constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling) |
| 135233 | p35S::HY5 | constitutive expression of HY5 from A. thaliana (AT5G11260; downregulates UPR signaling) |
| 135234 | p35S::mCh-HA-P2A-lifeact-mCh | constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid) |