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An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome.

Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, Fang H
PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
135188pBDC Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
135190pBDKIntegration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
135191pCNAArabinose inducible expression of lamba-Red recombinase proteins and I-CreI.
135192pSNAArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette
135193pSNKArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

Antibodies from Article