Engineered Reader Proteins for Enhanced Detection of Methylated Lysine on Histones.
Albanese KI, Krone MW, Petell CJ, Parker MM, Strahl BD, Brustad EM, Waters ML
ACS Chem Biol. 2020 Jan 17;15(1):103-111. doi: 10.1021/acschembio.9b00651. Epub 2019 Nov 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135089 | pET28a-MBP-CBX1-eReader | Bacterial expression of engineered CBX1 with enhanced binding to H3K9me |
| 135090 | pET28a-MBP-(CBX1)3-eReader | Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me |
| 180212 | pET28a-MBP-(CBX1)3-WT | Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me |