Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor.
O'Shaughnessy EC, Stone OJ, LaFosse PK, Azoitei ML, Tsygankov D, Heddleston JM, Legant WR, Wittchen ES, Burridge K, Elston TC, Betzig E, Chew TL, Adalsteinsson D, Hahn KM
J Cell Biol. 2019 Sep 2;218(9):3153-3160. doi: 10.1083/jcb.201903019. Epub 2019 Aug 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134926 | Rap1A FLARE | Rap1A biosensor |
| 134927 | Rap1B FLARE | Rap1B biosensor |
| 134928 | mCerulean3-Rap1 | Fluorescently tagged GTPase. |
| 134929 | RalGDS-YPet | Fluorescently tagged affinity reagent |