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Branchpoint translocation by fork remodelers as a general mechanism of R-loop removal.

Hodson C, van Twest S, Dylewska M, O'Rourke JJ, Tan W, Murphy VJ, Walia M, Abbouche L, Nieminuszczy J, Dunn E, Bythell-Douglas R, Heierhorst J, Niedzwiedz W, Deans AJ
Cell Rep. 2022 Dec 6;41(10):111749. doi: 10.1016/j.celrep.2022.111749. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
134899pUC19-Mu-switch-R-loopGeneration and purification of R-loops for in vitro biochemistry
134900pUC19-mAirn-R-loopGeneration and purification of R-loops for in vitro biochemistry
134901pUC19-hAPOE-R-loopGeneration and purification of R-loops for in vitro biochemistry
134902pUC19-hSNRPN-R-loopGeneration and purification of R-loops for in vitro biochemistry
134903pFL-EGFP-3xFlag-DESTBaculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag
193055pFL-EGFP-3xFlag-HLTFBaculovirus expression of human HLTF for recombinant protein production
193056pADC10-SETXhelBaculovirus expression of human Senataxin helicase domain for protein production
193058pFL-EGFP-3xFlag-Fml1Baculovirus expression of S.pombe Fml1 for recombinant protein production

Antibodies from Article