Multiplex suppression of four quadruplet codons via tRNA directed evolution.
DeBenedictis EA, Carver GD, Chung CZ, Soll D, Badran AH
Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134787 | pED1a6 | Luciferase reporter for quadruplet codon TAGA |
| 134788 | pED1a20 | Luciferase reporter for quadruplet codon CAGG |
| 134789 | pED1a34 | Luciferase reporter for quadruplet codon AGGG |
| 134790 | pED1a36 | Luciferase reporter for quadruplet codon CGGT |
| 134791 | pED1a39 | Luciferase reporter for quadruplet codon TACA |
| 134792 | pED1a71 | Luciferase reporter for quadruplet codon AGGA |
| 134793 | pED1a89 | Luciferase reporter for quadruplet codon CGGC |
| 134794 | pED14xQ-1 | IPTG inducible expression of Q-TAGA qtRNA |
| 134795 | pED14xQ-3 | IPTG inducible expression of Q-TAGA-Evo1 qtRNA |
| 134796 | pED14xQ-4 | IPTG inducible expression of Q-TAGA-Evo2 qtRNA |
| 134797 | pED14xR-1 | IPTG inducible expression of R-TAGA qtRNA |
| 134798 | pED14xR-3 | IPTG inducible expression of R-TAGA-Evo1 qtRNA |
| 134799 | pED14xR-4 | IPTG inducible expression of R-TAGA-Evo2 qtRNA |
| 134800 | pED14xS-1 | IPTG inducible expression of S-TAGA qtRNA |
| 134801 | pED14xS-3 | IPTG inducible expression of S-TAGA-Evo2 qtRNA |
| 134802 | pED14xS-4 | IPTG inducible expression of S-TAGA-Evo3 qtRNA |
| 134803 | pED14xS-11 | IPTG inducible expression of S-TAGA-Evo1 qtRNA |
| 134804 | pED14xS-WT | IPTG inducible expression of WT S tRNA scaffold tRNA |
| 134805 | pED14xW-1 | IPTG inducible expression of W-TAGA qtRNA |
| 134806 | pED14xW-3 | IPTG inducible expression of W-TAGA-Evo1 qtRNA |
| 134807 | pED14xY-1 | IPTG inducible expression of Y-TAGA qtRNA |
| 134808 | pED14xY-3 | IPTG inducible expression of Y-TAGA-Evo1 qtRNA |
| 134812 | pED7x10 | Lenient phage propagation reporter containing pIII-29-TAGA |
| 134813 | pED7x26 | Stringent phage propagation reporter containing pIII-16-29-34-TAGA |
| 134814 | pED17x11 | sfGFP expression plasmid for characterizing qtRNA charging |