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A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize.

Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
134745pVS1-VIR2Virulence and replication helper
134746pVS1-SAH2Replication helper
134748pG3GB411pGreen3 CRISPR/Cas9
134749pG3H-U3Ub pGreen3 CRISPR/Cas9
134750pG3B-U3UbpGreen3 CRISPR/Cas9
134751pCBC-MT1T2.2PCR template for sgRNA cloning
134752pCBC-DT1T2.2PCR template for sgRNA cloning
134753pG3B-U6SCpGreen3 CRISPR/Cas9
134754pG3K-U6SCpGreen3 CRISPR/Cas9
134755pG3H-U6SCpGreen3 CRISPR/Cas9
134756pG3B-U6EC1pGreen3 CRISPR/Cas9
134757pG3K-U6EC1pGreen3 CRISPR/Cas9
134758pG3H-U6EC1pGreen3 CRISPR/Cas9
134760pGB411pCambia CRISPR/Cas9
138192pVS1-VIR1Virulence helper
138193pRiA4-VIRVirulence helper compatible with pCambia binary vectors.

Antibodies from Article