Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products.
Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA
Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134672 | pET15b-tglHI | Expresses His6-TglH and TglI in E. Coli |
| 134673 | pRSFDuet1-tglA | Expresses His6-TglA in E. Coli |
| 134674 | pRSFDuet1-tglA(C51) | Expresses His6-TglA-Cys in E. Coli |
| 134675 | pRSFDuet1-tglA(C51)HIB | Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli |
| 134676 | pACYCDuet1-tglB | Expresses His6-TglBin E. Coli |
| 134677 | pACYCDuet1-tglCDEF | Expresses TglC, TglD, TglE, TglF in E. Coli |
| 134679 | pET28b-GFP-tglG | Expresses His6-GFP-TglG fusion in E. Coli |
| 134681 | pET28b-tglF | Expresses His6-TglF in E. Coli |
| 134682 | pACYCDuet1-ammA | Expresses His6-AmmA in E. Coli |
| 134683 | pACYCDuet1-ammA(∆Trp56) | Expresses His6-AmmA(∆Trp56) in E. Coli |
| 134684 | pACYCDuet1-ammA(∆50-56) | Expresses His6-AmmA(∆50-56) in E. Coli |
| 134685 | pET28a-ammB1 | Expresses His6-AmmB1 in E. Coli |
| 134686 | pET28a-ammB2 | Expresses His6-AmmB2 in E. Coli |
| 134687 | pET28a-ammB3 | Expresses His6-AmmB3 in E. Coli |
| 134688 | pET28a-ammB4 | Expresses His6-AmmB4 in E. Coli |