UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis.
Wang L, Xu Y, Rogers H, Saidi L, Noguchi CT, Li H, Yewdell JW, Guydosh NR, Ye Y
Cell Res. 2020 Jan;30(1):5-20. doi: 10.1038/s41422-019-0236-6. Epub 2019 Oct 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133861 | ER_K20 | Expression of ER-targeted Ribosome stalling reporter |
| 133862 | Cyto_K20 | Expression of cytoplasmic Ribosome stalling reporter |
| 133863 | ER_K0 | Expression of ER-targeted Ribosome non-stalling reporter |
| 133864 | Cyto_K0 | Expression of cytoplasmic Ribosome non-stalling reporter |
| 133865 | pRK5-Ufm1 WT | Expression of human wild type UFM1 |
| 133866 | pRK5-Ufm1 ∆C3 | Expression of human UFM1 mutant lacking the last three C-terminal residues |
| 133867 | pCMV6- Entry-RPL26DC-DDK | Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites |
| 134633 | px330-UFM1 sgRNA1 | contains sgRNA targeting human UFM1 for gene knockout |
| 134634 | px330-UFM1 sgRNA2 | contains sgRNA targeting human UFM1 for gene knockout |
| 134635 | px330-UFL1 sgRNA1 | contains sgRNA targeting human UFL1 for gene knockout |
| 134636 | px330-UFL1 sgRNA2 | contains sgRNA targeting human UFL1 for gene knockout |
| 134637 | px330-UFSP2 sgRNA2 | contains sgRNA targeting human UFSP2 for gene knockout |
| 134638 | px330-UFSP2 sgRNA1 | contains sgRNA targeting human UFSP2 for gene knockout |
| 134639 | pRK5-HA-UFM1 | expression of HA-tagged human UFM1 wildtype |
| 134640 | pRK5-FLAG-UFM1 | Expression of FLAG-tagged human UFM1 wildtype |
| 134641 | px330-RPL26 sgRNA1 | contains sgRNA targeting to the C-terminus of human RPL26 for gene editing |
| 134642 | px330-RPL26 sgRNA2 | contains sgRNA targeting to the C-terminus of human RPL26 for gene editing |