Dimerisation of APOBEC1 is dispensable for its RNA editing activity
Chieca M, Montini M, Severi F, Pecori R, Conticello S
bioRxiv 2021
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
112860 | human ACF | plasmid to overexpress human APOBEC1-complementation factor (ACF; A1CF) in mammalian cells |
112861 | rat APOBEC1 E63A | plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells |
134606 | pBlueScript-humanAPOBEC1 | Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity |
134607 | pEGFP-N1-Flag-humanRBM47 | Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays. |
167169 | pEGFP ratAPOBEC1 L173A/G227A | Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP. |
167171 | pEGFP humanAPOBEC1 L173A/G227A | Expresses human APOBEC1 L173A/G227A in mammalian cells |