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CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection.

Chen S, Sun S, Moonen D, Lee C, Lee AY, Schaffer DV, He L
Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
135616pscAAV-TyrDonor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus
135617pscAAV-Sox2-mStrDonor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus
135618pAAV-Sox2-CreERT2Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus
135619pAAV-R26-FLEX-mStrDonor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

Antibodies from Article