Awakening dormant glycosyltransferases in CHO cells with CRISPRa.
Karottki KJC, Hefzi H, Xiong K, Shamie I, Hansen AH, Li S, Pedersen LE, Li S, Lee JS, Lee GM, Kildegaard HF, Lewis NE
Biotechnol Bioeng. 2020 Feb;117(2):593-598. doi: 10.1002/bit.27199. Epub 2019 Nov 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134601 | VPR_dCas9 | Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker |