Transportable, Chemical Genetic Methodology for the Small Molecule-Mediated Inhibition of Heat Shock Factor 1.
Moore CL, Dewal MB, Nekongo EE, Santiago S, Lu NB, Levine SS, Shoulders MD
ACS Chem Biol. 2016 Jan 15;11(1):200-10. doi: 10.1021/acschembio.5b00740. Epub 2015 Nov 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134724 | pENTR1A dn-cHSF1 | Gateway entry vector for constitutively active dominant-negative HSF1 |
| 134726 | pENTR1A DHFR.dn-cHSF1 | Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain |
| 134728 | pLenti CMV Puro dn-cHSF1 | Lentiviral expression vector for constitutively active dominant-negative HSF1 |
| 134729 | pLenti CMV Puro DHFR.dn-cHSF1 | Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain |
| 134731 | pLenti CMV Hygro DHFR.dn-cHSF1 | Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain |
| 134732 | pLenti CMV Hygro dn-cHSF1 | Lentiviral expression vector for constitutively active dominant-negative HSF1 |
| 134734 | pLenti CMV/TO Zeo dn-cHSF1 | Lentiviral expression vector for inducible expression of a constitutively active dominant-negative HSF1 |
| 134735 | pDest40 DHFR.dn-cHSF1 | Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain |
| 134736 | pT-Rex Dest30 dn-cHSF1 | Mammalian expression vector for inducible expression of a constitutively active dominant-negative HSF1 |