A suite of PCR-based peptide tagging plasmids to permit epitope-targeted enzymatic functionalization of yeast proteins.
Nemec AA, Tomko RJ Jr
Yeast. 2020 May 13. doi: 10.1002/yea.3471.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134425 | pFA6a-A1:hphMX4 | PCR-based C-terminal A1 tag |
| 134426 | pFA6a-A1:kanMX6 | PCR-based C-terminal A1 tag |
| 134427 | pFA6a-A1:HIS3MX6 | PCR-based C-terminal A1 tag |
| 134428 | pFA6a-A1:natMX4 | PCR-based C-terminal A1 tag |
| 134429 | pFA6a-A1-FLAG:hphMX4 | PCR-based C-terminal A1-FLAG tag |
| 134430 | pFA6a-A1-FLAG:kanMX6 | PCR-based C-terminal A1-FLAG tag |
| 134431 | pFA6a-A1-FLAG:HIS3MX6 | PCR-based C-terminal A1-FLAG tag |
| 134432 | pFA6a-A1-FLAG:natMX4 | PCR-based C-terminal A1-FLAG tag |
| 134433 | pFA6a-S6:hphMX4 | PCR-based C-terminal S6 tag |
| 134434 | pFA6a-S6:kanMX6 | PCR-based C-terminal S6 tag |
| 134435 | pFA6a-S6:HIS3MX6 | PCR-based C-terminal S6 tag |
| 134436 | pFA6a-S6:natMX4 | PCR-based C-terminal S6 tag |
| 134437 | pFA6a-S6-FLAG:hphMX4 | PCR-based C-terminal S6-FLAG tag |
| 134438 | pFA6a-S6-FLAG:kanMX6 | PCR-based C-terminal S6-FLAG tag |
| 134439 | pFA6a-S6-FLAG:HIS3MX6 | PCR-based C-terminal S6-FLAG tag |
| 134440 | pFA6a-S6-FLAG:natMX4 | PCR-based C-terminal S6-FLAG tag |
| 134441 | pFA6a-SRS:hphMX4 | PCR-based C-terminal SRS tag |
| 134442 | pFA6a-SRS:kanMX6 | PCR-based C-terminal SRS tag |
| 134443 | pFA6a-SRS:HIS3MX6 | PCR-based C-terminal SRS tag |
| 134444 | pFA6a-SRS:natMX4 | PCR-based C-terminal SRS tag |
| 134445 | pFA6a-SRS-FLAG:hphMX4 | PCR-based C-terminal SRS-FLAG tag |
| 134446 | pFA6a-SRS-FLAG:kanMX6 | PCR-based C-terminal SRS-FLAG tag |
| 134447 | pFA6a-SRS-FLAG:HIS3MX6 | PCR-based C-terminal SRS-FLAG tag |
| 134448 | pFA6a-SRS-FLAG:natMX4 | PCR-based C-terminal SRS-FLAG tag |
| 145376 | pET28b-6His-AcpS | Expression of E. coli AcpS |