Light-Inducible Recombinases for Bacterial Optogenetics.

Sheets MB, Wong WW, Dunlop MJ
ACS Synth Biol. 2020 Feb 21;9(2):227-235. doi: 10.1021/acssynbio.9b00395. Epub 2020 Jan 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
134404	pBbE5a-Opto-Cre-Vvd	Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.
134405	pBbAW4k-loxP-TT-loxP-mRFP1	Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.
160400	pBbS5a-Opto-Cre-Vvd-2	Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.