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Accelerated evolution of a minimal 63-amino acid dual transcription factor.

Brodel AK, Rodrigues R, Jaramillo A, Isalan M
Sci Adv. 2020 Jun 10;6(24):eaba2728. doi: 10.1126/sciadv.aba2728. eCollection 2020 Jun. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
134351M13KO7-ΔPS-ΔgeneIIIHelper phage for M13 phage production
134352pLITMUS*-PBBa_J23106-geneIIIPhagemid
134353pLITMUS*-PrpoN-Cro-PBBa_J23106-geneIIIPhagemid
134354pLITMUS*-PrpoN-CroAct3-PBBa_J23106-geneIIIPhagemid
134355pLITMUS*-PrpoN-CroAct3 63aa-PBBa_J23106-geneIIIPhagemid
134356pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVIAccessory plasmid
134357pJPC12-ΔPS-mCherry-PCS/PM,CS-GFPReporter plasmid

Antibodies from Article