Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer.
Mahendra C, Christie KA, Osuna BA, Pinilla-Redondo R, Kleinstiver BP, Bondy-Denomy J
Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133788 | pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426) | CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40) |
| 133789 | pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579) | CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40) |
| 133790 | pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409) | CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40) |
| 133791 | pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14) | U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018 |
| 133792 | pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27) | U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015 |
| 133793 | pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482) | U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017 |
| 133794 | pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32) | U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018 |
| 133801 | pCMV-T7-AcrIIA4-NLS(SV40) (KAC200) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40) |
| 133802 | pCMV-T7-AcrIIA5-NLS(SV40) (KAC203) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40) |
| 134331 | pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582) | CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag |
| 134332 | pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40) |
| 134333 | pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40) |
| 134334 | pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40) |
| 134335 | pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40) |
| 134336 | pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40) |
| 134337 | pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40) |
| 134338 | pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40) |
| 134339 | pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40) |
| 134340 | pCMV-T7-AcrIIC1-NLS(SV40) (KAC208) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40) |
| 134341 | pCMV-T7-AcrIIC3-NLS(SV40) (KAC209) | CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40) |