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MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysis.

Yuno M, Nagata S, Fujita T, Fujii H
Biol Methods Protoc. 2021 Jul 9;6(1):bpab013. doi: 10.1093/biomethods/bpab013. eCollection 2021. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
1343233xFLAG-dCas9/pMSCVhygExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
1349823xFLAG-dCas9/pMSCVneoExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
1349833xFLAG-dCas9/pMSCVpuroExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
135392gRNA-hIRF-1 #12/pSIR-hCD2Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Antibodies from Article