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PML is recruited to heterochromatin during S phase and represses DAXX-mediated histone H3.3 chromatin assembly.

Shastrula PK, Sierra I, Deng Z, Keeney F, Hayden JE, Lieberman PM, Janicki SM
J Cell Sci. 2019 Mar 26;132(6). pii: jcs.220970. doi: 10.1242/jcs.220970. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
134524YFP-DAXX-mSIM1Expresses YFP-DAXX fusion protein with a mutation in its 5' SIM
134525YFP-DAXX-mSIM1/2Expresses YFP-DAXX fusion protein with a mutations in both its 5' and 3' SIMs
134529YFP-PML-IV-mSIM-C3Expresses Cherry-PML-IV fusion protein with a mutation in its SIM

Antibodies from Article