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Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential.

Swartz SZ, McKay LS, Su KC, Bury L, Padeganeh A, Maddox PS, Knouse KA, Cheeseman IM
Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi: 10.1016/j.devcel.2019.07.016. Epub 2019 Aug 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
134233pZS039For in vitro transcription of Miniata CENP-A
134235pZS077Bacterial expression of CENP-C
134238pZS160Lentiviral plasmid expressing sgRNA against HJURP
134240pKC123expresses gRNA targeting CENP A 3'
134242pKC158CENP-A Halo expression construct
134243pKM328Mammalian expression construct expressing sgRNA against PLK1
134244pLM032Retroviral plasmid with H3.1A EGFP insert
134245pLM033Retroviral plasmid with H4 EGFP insert

Antibodies from Article