Microtubule Tip Tracking by the Spindle and Kinetochore Protein Ska1 Requires Diverse Tubulin-Interacting Surfaces.
Monda JK, Whitney IP, Tarasovetc EV, Wilson-Kubalek E, Milligan RA, Grishchuk EL, Cheeseman IM
Curr Biol. 2017 Dec 4;27(23):3666-3675.e6. doi: 10.1016/j.cub.2017.10.018. Epub 2017 Nov 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134125 | pIW028 | Bacterial expression of human Ska1 microtubule binding domain (MTBD) |
| 134126 | pIW039 | Bacterial expression of human Ska1 microtubule binding domain (MTBD) |
| 134127 | pIW040 | Bacterial expression of human Ska1 microtubule binding domain (MTBD) |
| 134128 | pIW087 | Bacterial expression of GFP-Ska1 and Ska2 |
| 134129 | pIW090 | Bacterial expression of GFP-Ska1 and Ska2 |
| 134130 | pIW084 | Bacterial expression of GFP-Ska1 and Ska2 |
| 134131 | pIW095 | Bacterial expression of GFP-Ska1 and Ska2 |
| 134132 | pIW086 | Bacterial expression of His-TEV-Ska1 and Ska2 |
| 134133 | pIW069 | Retroviral expression of GFP-Ska1 |
| 134134 | pIW085 | Retroviral expression of GFP-Ska1 |
| 134135 | pIW088 | Retroviral expression of GFP-Ska1 |
| 134136 | pIW082 | Retroviral expression of GFP-Ska1 |
| 134137 | pIW093 | Retroviral expression of GFP-Ska1 |
| 134138 | pIW070 | Retroviral expression of GFP-Ska1 |
| 134139 | pJS278 | Bacterial expression of human Ska1 microtubule binding domain (MTBD) |
| 134141 | pJS263 | Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD) |
| 134142 | pJS183 | Bacterial expression of GFP-Ska1 and Ska2 |
| 134146 | pJS249 | Bacterial expression of human Ska1 microtubule binding domain (MTBD) |
| 134147 | pCB004 | Bacterial expression of human Ska3 |
| 134169 | pJM160 | Bacterial expression of RB3 stathmin-like domain (SLD) |
| 134202 | pDK616 | Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant |