High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803.
Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P
Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.1038/s41598-020-62681-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133970 | pSHDY*in_PpetE:BCD2:mVenus | Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module. |
| 133971 | pSHDY*in_PpetE:BCD2:AgBIS:Flag | Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag. |
| 133973 | pSHDY*in_PpetE:BCD2:MrBBS:Strep | Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag. |