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Efficient modification of lambda-DNA substrates for single-molecule studies.

Kim Y, de la Torre A, Leal AA, Finkelstein IJ
Sci Rep. 2017 May 18;7(1):2071. doi: 10.1038/s41598-017-01984-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133935pIF251Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.
134836IF189Rapid and efficient recombineering and purificiation of modified lambda phage DNA

Antibodies from Article