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Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria.

Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA
Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133920pJAB988miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

Antibodies from Article