Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins.
Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW
Nat Chem. 2020 Nov;12(11):1008-1015. doi: 10.1038/s41557-020-0528-y. Epub 2020 Sep 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133909 | pET28-His6-Ubc9 | Bacterial expression of His6-Ubc9 |
| 133910 | pET28-His6-Ubc9-C93A | Bacterial expression of His6-Ubc9-C93A |
| 133911 | pET28-His6-Ubc9-C138A | Bacterial expression of His6-Ubc9-C138A |
| 133913 | pET28-His6-GFP-C-LACE | Bacterial expression of His6-GFP-C-LACE |