Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin.

Courtney NA, Bao H, Briguglio JS, Chapman ER
Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
133815	pFSW GFP IRES GFP	Encodes EGFP that can be used as a control for viral expression
133816	pFSW Syt1 WT IRES GFP	Encodes full-length wild type synaptotagmin 1 for viral expression
133817	pFSW Syt1-C2B IRES GFP	Encodes the C2B domain of synaptotagmin 1 for viral expression
133818	pFSW Syt1-C2A IRES GFP	Encodes the C2A domain of synaptotagmin 1 for viral expression
133819	pFSW Syt1-C2B R398,399Q IRES GFP	Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression
133820	pFSW Syt1-C2B K326,327E IRES GFP	Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression
133821	pFSW Syt1-C2B V304A,I367A IRES GFP	Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression
133822	pFSW Syt1-C2B D363,365N IRES GFP	Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression
133823	pFSW Syt1-C2A K189-192A IRES GFP	Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression
133824	pFSW Syt1-C2A D230,232N IRES GFP	Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression
133825	pFSW Syt1-C2A M173,F234A IRES GFP	Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression