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Listeria Phages Induce Cas9 Degradation to Protect Lysogenic Genomes.

Osuna BA, Karambelkar S, Mahendra C, Christie KA, Garcia B, Davidson AR, Kleinstiver BP, Kilcher S, Bondy-Denomy J
Cell Host Microbe. 2020 Apr 18. pii: S1931-3128(20)30190-6. doi: 10.1016/j.chom.2020.04.001. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133795pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)
133796pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)
133797pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)
133798pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)
133799pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)
133800pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

Antibodies from Article