Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells.
Viswanatha R, Li Z, Hu Y, Perrimon N
Elife. 2018 Jul 27;7. pii: 36333. doi: 10.7554/eLife.36333.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133781 | pMK33/Cas9 | Expresses drosophila-optimized Cas9 |
| 133782 | pMK33/intein-Cas9_S219-3XFLAG | Expresses human codon-optimized intein-Cas9 |
| 133783 | pLib6.4 | Library backbone |
| 134582 | Drosophila_sgRNA_group_1 | Targets kinases, phosphatases, and Drosophila orthologs of drug targets |
| 134583 | Drosophila_sgRNA_group_2 | Targets Drosophila orthologs of vertebrate genes that have undergone expansion in the vertebrate line |
| 134584 | Drosophila_sgRNA_group_3 | Targets all other Drosophila genes not included in Groups 1 and 2 |