SPARC enables genetic manipulation of precise proportions of cells.
Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin TR
Nat Neurosci. 2020 Sep;23(9):1168-1175. doi: 10.1038/s41593-020-0668-9. Epub 2020 Jul 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133559 | pHD-3xP3-dsRed-DattP | Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs |
| 133560 | pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 | Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus |
| 133561 | pCFD5-U6-3-t-attP40 | gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs |
| 133562 | pHD-SPARC2-D-LexA::p65 | Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus |
| 133563 | pHD-SPARC2-I-LexA::p65 | Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus |
| 133564 | pHD-SPARC2-S-LexA::p65 | Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus |
| 133565 | pJFRC-20XUAS-IVS-PhiC31 | Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest. |
| 133868 | pJFRC-nSyb-IVS-PhiC31 | Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest. |