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A method for cost-effective and rapid characterization of engineered T7-Based transcription factors by cell-free protein synthesis reveals insights into the regulation of T7 Rna polymerase-driven expression.

McManus JB, Lux MW, Murray RM, Emanuel PA
Arch Biochem Biophys. 2019 Jul 18. pii: S0003-9861(19)30288-7. doi: 10.1016/j.abb.2019.07.010. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133521pY71-tet.I.8sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site
133523pY71-tet.I.1sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site
133524pY71-tet.I.2sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site
133525pY71-tet.I.3sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site
133526pY71-tet.I.4sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site
133527pY71-tet.I.5sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site
133528pY71-tet.I.6sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site
133529pY71-tet.I.7sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site
133530pY71-tet.I.9sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site
133531pY71-tet.I.10sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site
133532pY71-tet.I.11sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site
133533pY71-tet.I.12sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site
133534pY71-tet.I.13sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site
133535pY71-tet.I.14sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site
133536pY71-tet.I.16sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site
133537pY71-tet.I.20sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site
133538pY71-tet.I.25sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site
133539pY71-tet.I.-1sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

Antibodies from Article