A toolbox of stable integration vectors in the fission yeast Schizosaccharomyces pombe.
Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, Billault-Chaumartin I, Martin SG
J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 10.1242/jcs.240754.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133467 | pAV0133 | Integration into fission yeast genome at ura4 locus |
| 133468 | pAV0356 | Integration into fission yeast genome at ade6 locus |
| 133469 | pAV0357 | Integration into fission yeast genome at lys3 locus |
| 133470 | pAV0413 | Integration into fission yeast genome at his5 locus |
| 133471 | pAV0584 | Integration into fission yeast genome at ura4 locus with natMX selection cassette |
| 133472 | pAV0585 | Integration into fission yeast genome at ade6 locus with hphMX selection cassette |
| 133473 | pAV0586 | Integration into fission yeast genome at lys3 locus with bsdMX selection cassette |
| 133474 | pAV0587 | Integration into fission yeast genome at his5 locus with bleMX selection cassette |
| 133475 | pAV0784 | Integration into fission yeast genome at his5 locus with bsdMX selection cassette |
| 133476 | pAV0661 | Expression in fission yeast from promoter pact1, integrated at ade6 locus |
| 133477 | pAV0662 | Expression in fission yeast from promoter pact1, integrated at lys3 locus |
| 133478 | pAV0663 | Expression in fission yeast from promoter pact1, integrated at his5 locus |
| 133479 | pAV0714 | Expression in fission yeast from promoter pact1, integrated at ura4 locus |
| 133480 | pAV0746 | Expression in fission yeast from promoter prga3 , integrated at ura4 locus |
| 133481 | pAV0747 | Expression in fission yeast from promoter ppom1 , integrated at ura4 locus |
| 133482 | pAV0748 | Expression in fission yeast from promoter ppak1 , integrated at ura4 locus |
| 133483 | pAV0749 | Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus |
| 133484 | pAV0750 | Expression in fission yeast from promoter purg1 , integrated at ura4 locus |
| 133485 | pAV0751 | Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus |
| 133486 | pAV0752 | Expression in fission yeast from promoter pnmt41 , integrated at ura4 locus |
| 133487 | pAV0753 | Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus |
| 133488 | pAV0327 | Expression of nucleo-cytoplasmic sfGFP |
| 133489 | pAV0328 | Expression of nucleo-cytoplasmic mCherry |
| 133490 | pAV0471 | Expression of nucleo-cytoplasmic mTagBFP2 |
| 133491 | pAV0624 | Expression of cytoplasmic 3mTagBFP2 |
| 133492 | pAV0517 | Expression of nucleo-cytoplasmic GFP in P-cells |
| 133493 | pAV0543 | Expression of nucleo-cytoplasmic mCherry in P-cells |
| 133494 | pAV0761 | Expression of nucleo-cytoplasmic mTagBFP2 in P-cells |
| 133495 | pAV0523 | Expression of nucleo-cytoplasmic sfGFP in M-cells |
| 133496 | pAV0762 | Expression of nucleo-cytoplasmic mCherry in M-cells |
| 133497 | pAV0763 | Expression of nucleo-cytoplasmic mTagBFP2 in M-cells |
| 133498 | pAV0478 | Expression of sfGFP targeted to the nucleus with two NLS signals |
| 133499 | pAV0479 | Expression of sfGFP excluded from the nucleus with two NES signals |
| 133500 | pAV0526 | Expression of sfGFP targeted to the nucleus with one NLS signals |
| 133501 | pAV0765 | Expression of mCherry targeted to the nucleus with one NLS signals |
| 133502 | pAV0532 | Expression of mTagBFP2 targeted to the nucleus with one NLS signals |
| 133503 | pAV0605 | Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC |
| 133504 | pAV0607 | Expression of mCherry targeted to the plasma membrane with an amphipathic helix of RitC |
| 133505 | pAV0612 | Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC |
| 133506 | pAV0620 | Expression of eGFP tagged PCNA to monitor DNA replication |
| 133507 | pAV0785 | Expression of mCherry tagged PCNA to monitor DNA replication |
| 133508 | pAV0756 | Expression of 3mCherry tagged CRIB domain to monitor active Cdc42 |
| 133509 | pAV0757 | Expression of 3GFP tagged CRIB domain to monitor active Cdc42 |
| 133510 | pAV0816 | Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42 |
| 133511 | pAV0758 | Expression of 3GFP tagged RasAct probe to monitor active Ras1 |
| 133512 | pAV0835 | Expression of mCherry tagged CHD domain to monitor actin cytoskeleton |
| 133513 | pAV0771 | Expression of mTagBFP2 tagged CHD domain to monitor actin cytoskeleton |
| 133514 | pAV0782 | Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton |
| 133515 | pAV0783 | Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton |
| 133516 | pAV0772 | Expression of sfGFP tagged beta-tubulin to monitor microtubules |
| 133517 | pAV0710 | Expression of mCherry tagged beta-tubulin to monitor microtubules |
| 133518 | pAV0770 | Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules |
| 133519 | pAV0773 | Expression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL) |
| 133520 | pAV0764 | Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL) |