Gerhard Wagner Lab: Wagner Lab plasmids

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
133421	pst39-NST-clone14	S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
133422	pGAPZa-STE2-1D4	Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
133423	pGAPZ-STE2-1D4	Yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
133424	pYES2-STE2-1D4	Mutant yeast STE2 coding sequence in vector for expression in yeast S. cerevisiae, with Gal1 promoter.
133427	pHIS-GB1-BP1	Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.
133430	pET-24a-STE2	Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
133431	pET-24a-CXCR4	Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
133433	pPICZaa-STE2-cohis	Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
133436	pPICZa-STE2-1D4	Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
133437	pPICZac-STE2-1D4	Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
133439	RG-001	Heat 3 domain of human elF4G in pET21 backbone.
133440	KE-005	Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
133441	KE-009	Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.