Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules.
Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS
Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133123 | pOGG043 | Level 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum. |
| 133124 | pOGG113 | pL0M-PU-NoP, promotorless region used as negative control |
| 133227 | pOPS0262 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133228 | pOPS0263 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133229 | pOPS0378 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133230 | pOPS0380 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133231 | pOPS0381 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133232 | pOPS0382 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133233 | pOPS0383 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133234 | pOPS0384 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133235 | pOPS0385 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |
| 133236 | pOPS0386 | Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present. |