Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics.
Chen SY, Osimiri LC, Chevalier M, Bugaj LJ, Nguyen TH, Greenstein RA, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H
Cell Syst. 2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 10.1016/j.cels.2020.08.009.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133085 | pCLASP_Crz1(19A)_CLASP_RGS2membrane | Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light. |
| 133086 | pCLASP_mScarlet_CLASP_RGS2membrane | Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl |
| 133118 | pYPS1-Venus | Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus. |
| 133119 | pCMK2-Venus | Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus. |
| 133120 | pGYP7-Venus | Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus. |