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Structural basis for the docking of mTORC1 on the lysosomal surface.

Rogala KB, Gu X, Kedir JF, Abu-Remaileh M, Bianchi LF, Bottino AMS, Dueholm R, Niehaus A, Overwijn D, Fils AP, Zhou SX, Leary D, Laqtom NN, Brignole EJ, Sabatini DM
Science. 2019 Oct 25;366(6464):468-475. doi: 10.1126/science.aay0166. Epub 2019 Oct 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133070pFloat.T7_v1Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.
133071pFloat.cspA_v1Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.
133072pDarmo.CMVT_v1Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.
133083pFloat.assemble(AB).LC_v1Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.
133084pFloat.assemble(AB).HC_v1Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

Antibodies from Article