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Mechanisms of Toll-like Receptor 4 Endocytosis Reveal a Common Immune-Evasion Strategy Used by Pathogenic and Commensal Bacteria.

Tan Y, Zanoni I, Cullen TW, Goodman AL, Kagan JC
Immunity. 2015 Nov 17;43(5):909-22. doi: 10.1016/j.immuni.2015.10.008. Epub 2015 Nov 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133061pMSCV-IRES-hCD2-CD14Retroviral expression of mouse CD14
133062pMSCV-IRES-hCD2-CD14 (4R)Retroviral expression of mouse CD14 mutant "4R" deficient in LPS binding
133063pMSCV-IRES-GFP-TLR4 delta tailRetroviral expression of mouse TLR4 with out its cytosolic tail
133064pMSCV-IRES-GFP-CD4-TLR4 cytoRetroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4
133065pMSCV-IRES-GFP-MD2-HARetroviral expression of mouse MD2 tagged with HA
133066pMSCV-IRES-GFP-MD2 (C95A)-HARetroviral expression of mouse MD2 (C95A) tagged with HA
133067pMSCV-IRES-GFP-MD2 (F126A)-HARetroviral expression of mouse MD2 (F126A) tagged with HA

Antibodies from Article