Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND
Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132968 | p5E-6xgata2aECEbasp | gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector |
| 132969 | p5E-6xgata2aECE | gata2a endothelial enhancer (x6) in p5E Gateway vector |
| 132970 | pCS-foxc1a-2a-nlstdTomato | full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector |
| 132971 | pME-foxc1a | full-length of foxc1a without stop codon in pmE Gateway vector |
| 132972 | pME-Kaede | full-length of photoconvertible kaede with stop codon in pmE Gateway vector |
| 132973 | pME-nls-sfGFP | full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector |
| 132974 | pTone-gata2aECE-EGFP | gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone |
| 132975 | pTone-gata2aECE-nsGFP | gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone |
| 132976 | pTone-gata2aECE-Kaede | gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone |
| 132977 | pME-gata2a | full-length of gata2a without stop codon in pmE Gateway vector |
| 132978 | pDR-nfatc1_sgRNA2 | nfatc1 sgRNA targeting to "5'-GTGGGAGCTCCATTGGATCG-3" in pDR274 |
| 132979 | pDR-itga9_sgRNA1 | itga9 sgRNA targeting to "5'-GAATGACGGAGCTCTCTACG-3" in pDR274 |
| 132980 | pDR-itga9_sgRNA4 | itga9 sgRNA targeting to "5'-TCCGCTGCCAGCCAGCCGG-3" in pDR274 |
| 132981 | pDR-itga9_sgRNA7 | itga9 sgRNA targeting to "5'-CCCTCCAGCCTTCCATATG-3" in pDR274 |
| 132985 | pCS-nlstdTomato | full length of nuclear localized tdTomato in pCS expression vector |