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CRISPR-Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference.

Zhao Y, Boeke JD
Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 10.1093/nar/gkaa329. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132952pYZ221crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombe
132953pYZ673crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe
132954pYZ675crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe
132955pYZ714crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe
132956pYZ694crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Antibodies from Article