CRISPR-Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference.
Zhao Y, Boeke JD
Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 10.1093/nar/gkaa329.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
132952 | pYZ221 | crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombe |
132953 | pYZ673 | crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe |
132954 | pYZ675 | crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe |
132955 | pYZ714 | crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe |
132956 | pYZ694 | crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe |