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Search-and-replace genome editing without double-strand breaks or donor DNA.

Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132774pCMV-PE1Prime editing in mammalian cells
132775pCMV-PE2Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b
132776pCMV-PE2-P2A-GFPPrime editing in mammalian cells with co-translational GFP expression
132777pU6-pegRNA-GG-acceptorPrime editing in mammalian cells
132778pU6-Sp-pegRNA-HEK3_CTT_insS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing
135955U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lentiNeuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
135956hSynapsin-PE2-Cterminal-lentiNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b
135957pU6-Sp-pegRNA-RNF2_+5GtoTS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing
135958pU6-sp-sgRNA-RNF2_+41nickS. pyogenes sgRNA for +41 nick in RNF2
135959pU6-sp-sgRNA-mDNMT1_nick-lentiS. pyogenes nicking sgRNA for mouse DNMT1

Antibodies from Article