De novo-designed translation-repressing riboregulators for multi-input cellular logic.
Kim J, Zhou Y, Carlson PD, Teichmann M, Chaudhary S, Simmel FC, Silver PA, Collins JJ, Lucks JB, Yin P, Green AA
Nat Chem Biol. 2019 Dec;15(12):1173-1182. doi: 10.1038/s41589-019-0388-1. Epub 2019 Nov 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132722 | pYZ_3WJrep_N20_switch | T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter |
| 132723 | pYZ_3WJrep_N19_switch | T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter |
| 132724 | pYZ_3WJrep_N10_switch | T7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporter |
| 132725 | pYZ_3WJrep_N24_switch | T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter |
| 132726 | pYZ_3WJrep_N20_trigger | T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA |
| 132727 | pYZ_3WJrep_N19_trigger | T7 RNAP-driven expression of 3WJ repressor index 19 trigger RNA |
| 132728 | pYZ_3WJrep_N10_trigger | T7 RNAP-driven expression of 3WJ repressor index 10 trigger RNA |
| 132729 | pYZ_3WJrep_N24_trigger | T7 RNAP-driven expression of 3WJ repressor index 24 trigger RNA |
| 132730 | pYZ_NAND2_L17_S19_S11 | T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11 |
| 132731 | pYZ_NAND3_L11_S11_S13_S19 | T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19 |
| 132732 | pYZ_NAND4_L17_S24_S11_S19_S13 | T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13 |
| 132733 | pYZ_3WJrep_N11_trigger | T7 RNAP-driven expression of 3WJ repressor index 11 trigger RNA |
| 132734 | pYZ_3WJrep_N12_trigger | T7 RNAP-driven expression of 3WJ repressor index 12 trigger RNA |
| 132735 | pYZ_3WJrep_N13_trigger | T7 RNAP-driven expression of 3WJ repressor index 13 trigger RNA |
| 132736 | pAG_PluxB_ToeRep_N01_trigger | Inducible expression of first-generation toehold repressor index 1 trigger RNA via the PluxB promoter |
| 132737 | pAG_Ptet*_ToeRep_N01_switch | Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter |
| 132738 | pYZ_PluxB_3WJrep_N19_trigger | Inducible expression of 3WJ repressor index 19 trigger RNA via the PluxB promoter |
| 132739 | pYZ_Ptet*_3WJrep_N19_switch | Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter |
| 132740 | pAG_ToeRep_N09_trigger | T7 RNAP-driven expression of first-generation toehold repressor index 9 trigger RNA |
| 132741 | pAG_ToeRep_N09_switch | T7 RNAP-driven expression of first-generation toehold repressor index 9 switch RNA with a GFP-ASV reporter |
| 132742 | pJK_ToeRepG2_N02_switch | T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter |
| 132743 | pJK_ToeRepG2_N64_switch | T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter |
| 132744 | pJK_ToeRepG2_N19_switch | T7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporter |
| 132745 | pJK_ToeRepG2_N02_trigger | T7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNA |
| 132746 | pJK_ToeRepG2_N64_trigger | T7 RNAP-driven expression of second-generation toehold repressor index 64 trigger RNA |
| 132747 | pJK_ToeRepG2_N19_trigger | T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA |