DABs are inorganic carbon pumps found throughout prokaryotic phyla.
Desmarais JJ, Flamholz AI, Blikstad C, Dugan EJ, Laughlin TG, Oltrogge LM, Chen AW, Wetmore K, Diamond S, Wang JY, Savage DF
Nat Microbiol. 2019 Aug 12. pii: 10.1038/s41564-019-0520-8. doi: 10.1038/s41564-019-0520-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132998 | pFE-sfGFP | pFE carrying a sfGFP gene |
| 132999 | pFE-hCA | pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918) |
| 133000 | pFE-dabB2 | pFE carrying a dabB2 gene (Uniprot: D0KWS8) |
| 133001 | pFE-dabA2 | pFE carrying a dabA2 gene (Uniprot: D0KWS7) |
| 133002 | pFE-dabAB2 | pFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8) |
| 133003 | pFE-baDAB | pFE carrying the badabA and badabB genes |
| 133004 | pFE-vcDAB | pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955) |
| 133005 | pFE-dabAB2 C351A | pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8) |
| 133006 | pFE-dabAB2 D353A | pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene (Uniprot: D0KWS8) |
| 133007 | pFE-dabAB2 H524A | pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8) |
| 133008 | pFE-dabAB2 C539A | pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene (Uniprot: D0KWS8) |
| 133009 | pFA-GFP | pFA carrying a sfGFP gene |
| 133010 | pFA-eCA | pFA carrying a E. coli canA gene (Uniprot: P61517) |
| 133011 | pFA-sbtA | pFA carrying a Synechococcus elongatus PCC 7942 sbtA gene (Uniprot: Q6UP27) |
| 133012 | pFA-dabAB2 | pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959) |
| 133013 | pET14b-dabAB2 | pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag |
| 133014 | pET14b-dabAB2 C351A | pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag |
| 133016 | pET14b-dabAB2 D353A | pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag |