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Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting.

Bishe B, Taton A, Golden JW
iScience. 2019 Oct 25;20:216-228. doi: 10.1016/j.isci.2019.09.002. Epub 2019 Sep 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132660pAM5404RSF1010-based broad host-range plasmid with additional RK2 bom site
132661pAM5406RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin
132662pAM5409RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance
132663pAM5411RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance
132664pAM5505pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Antibodies from Article