Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells.
Wierson WA, Simone BW, WareJoncas Z, Mann C, Welker JM, Kar B, Emch MJ, Friedberg I, Gendron WAC, Barry MA, Clark KJ, Dobbs DL, McGrail MA, Ekker SC, Essner JJ
CRISPR J. 2019 Dec;2(6):417-433. doi: 10.1089/crispr.2019.0026. Epub 2019 Nov 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132640 | pMini-CAAGs::RFP-DR48 | Fluorescent reporter to quantify elicitation of the strand annealing mediated repair pathway |
| 132641 | pErCas12a EZ Clone | Expresses ErCas12a and an sgRNA to target a region of interest |
| 132642 | pT3TS nErCas12an | Used for in vitro transcription of dual NLS ErCas12a mRNA |