Antimicrobial solid media for screening non-sterile Arabidopsis thaliana seeds.
Behrendorff JBYH, Borras-Gas G, Pribil M
Physiol Plant. 2020 Feb 25. doi: 10.1111/ppl.13079.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132565 | pN_35S/mEGFP | mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat) |
| 132566 | pN_35S/mApple | mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat) |
| 132568 | GS2.1/EC | sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt). |
| 135320 | pB_35S/mEGFP | mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar) |
| 135321 | pH_35S/mEGFP | mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt) |