Structure of the dynein-2 complex and its assembly with intraflagellar transport trains.
Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, Roberts AJ
Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132524 | His-ZZ-TEV-SNAPf-DYNC2H1 | Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments |
| 132525 | WDR60 | Human WDR60, codon optimised for Sf9 expression. |
| 132526 | WDR34 | Human WDR34, codon optimised for Sf9 expression. |
| 132527 | DYNC2LI1 | Human LIC3, codon optimised for Sf9 expression. |
| 132528 | DYNLT1 | Human TCTEX-1, codon optimised for Sf9 expression. |
| 132529 | TCTEX1D2 | Human TCTEC-1D2, codon optimised for Sf9 expression. |
| 132530 | DYNLL1 | Human LC8-1, codon optimised for Sf9 expression. |
| 132531 | DYNLL2 | Human LC8-2, codon optimised for Sf9 expression. |
| 132532 | DYNLT3 | Human TCTEX-3, codon optimised for Sf9 expression. |
| 132533 | DYNLRB1 | Human Rbl-1, codon optimised for Sf9 expression. |
| 132534 | DYNLRB2 | Human Rbl-2, codon optimised for Sf9 expression. |
| 132536 | Dynein-2_complex | Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like. |
| 133115 | LC8-like | Human LC8-like protein, codon optimised for Sf9 expression. |