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The repurposing of type I-E CRISPR-Cascade for gene activation in plants.

Young JK, Gasior SL, Jones S, Wang L, Navarro P, Vickroy B, Barrangou R
Commun Biol. 2019 Oct 18;2:383. doi: 10.1038/s42003-019-0637-6. eCollection 2019. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132334PV225dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
132335PV244CasB constitutive expression cassette for Zea mays
132336PV245CasC constitutive expression cassette for Zea mays
132337PV246CasE constitutive expression cassette for Zea mays
132338PV247CasA constitutive expression cassette for Zea mays
132339PV248CasD constitutive expression cassette for Zea mays
132340PV259CasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
132342PV503CasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
132343PV504CasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
132344PV384Cas9 DsRed targeting sgRNA expression cassette for Zea mays
132345PV422SthCascade DsRed targeting crRNA expression cassette for Zea mays
132346PV521Cas9 R promoter targeting sgRNA1 expression cassette for Zea mays
132347PV523Cas9 R promoter targeting sgRNA2 expression cassette for Zea mays
132348PV528Cas9 R promoter targeting sgRNA3 expression cassette for Zea mays
132349PV564SthCascade R promoter targeting crRNA1 expression cassette for Zea mays
132350PV565SthCascade R promoter targeting crRNA2 expression cassette for Zea mays
132351PV569SthCascade R promoter targeting crRNA3 expression cassette for Zea mays
132352PHP665C1 over-expression cassette for Zea mays
132353PHP11004R over-expression cassette for Zea mays

Antibodies from Article