DNA variants affecting the expression of numerous genes in trans have diverse mechanisms of action and evolutionary histories.
Lutz S, Brion C, Kliebhan M, Albert FW
PLoS Genet. 2019 Nov 18;15(11):e1008375. doi: 10.1371/journal.pgen.1008375. eCollection 2019 Nov.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131774 | pFA0055 | Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles. |
| 131784 | pFA0057 | Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles. |