Efficient and flexible tagging of endogenous genes by homology-independent intron targeting.
Serebrenik YV, Sansbury SE, Kumar SS, Henao-Mejia J, Shalem O
Genome Res. 2019 Aug;29(8):1322-1328. doi: 10.1101/gr.246413.118. Epub 2019 Jun 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184162 | pMC_mNG2(11)_BSDminus_F0 | Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants. |
| 184163 | pMC_mNG2(11)_BSDminus_F1 | Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants. |
| 184164 | pMC_mNG2(11)_BSDminus_F2 | Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants. |
| 184165 | pMC_mClover3_BSDminus_F0 | Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants. |
| 184166 | pMC_mClover3_BSDminus_F1 | Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants. |
| 184167 | pMC_mClover3_BSDminus_F2 | Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants. |
| 184168 | sgDonor_lentiGuide | LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid. |
| 184169 | mNG2(1-10)_tdTomato | Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein. |