CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1.
Naduthodi MIS, Mohanraju P, Sudfeld C, D'Adamo S, Barbosa MJ, van der Oost J
Biotechnol Biofuels. 2019 Mar 25;12:66. doi: 10.1186/s13068-019-1401-3. eCollection 2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154347 | pNAD1 | pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance |